Development of a novel tissue adhesive using a naturally-derived small molecule
نویسندگان
چکیده
A citric acid derivative (CAD) with three active ester groups in a molecule was prepared in order to develop a novel tissue adhesive consisting of CAD and collagen (CAD-C glue). The bonding strength of CAD-C glue to porcine soft tissues had high value similar to the tearing strength of the original soft tissue. The cytotoxicity of CAD for L929 fibroblast was 10 times less cytotoxic than that of glutaraldehyde and formaldehyde that are used in gelatin-resorcinol-formaldehyde glue. This adhesive developed will be applied as bonding reagent for soft tissues. Introduction In order to bond tissue-tissue interface with high bonding strength, synthetic or semi-synthetic tissue adhesives, such as cyanoacrylate-based glue, gelatin-resorcinol-formaldehyde (GRF) glue, and other glues have been developed and applied for biomedical field. However, GRF glue and cyanoacrylate-glue cause chronic inflammation and delay wound healing due to the residual reaction products or degradation products such as glutaraldehyde and formaldehyde. Therefore, fibrin glue 7 that is based on the polymerization reaction between fibrinogen and thrombin has been widely used in clinical applications. Fibrin glue has low cytotoxicity for human body and promotes wound healing, however, its low bonding strength to tissues limits the use in medical field. In order to adhere tissues with low cytotoxicity and high bonding strength, some researchers have tried to use various crosslinking reagents including genipin, diepoxy compounds and carbodiimide. These crosslinking reagents are reported to be much lower cytotoxicity than aldehyde compounds. N-hydroxysuccinimide (NHS) poly(L-glutamic acid) esters were also reported to crosslink gelatin and adhere tissues with low cytotoxicity. However, these adhesives have not yet had sufficient bonding strength. Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. 1. REPORT DATE 00 JUN 2003 2. REPORT TYPE N/A 3. DATES COVERED 4. TITLE AND SUBTITLE Development of a novel tissue adhesive using a naturally-derived small molecule 5a. CONTRACT NUMBER
منابع مشابه
Kojic acid-derived tyrosinase inhibitors: synthesis and bioactivity
Tyrosinase is a key enzyme for melanin biosynthesis, catalyzing the oxidation of L-tyrosine to L-dopaquinone. The tyrosinase inhibition is an effective approach to control hyperpigmentation in human skin and enzymatic browning in fruits and vegetables. Kojic acid is a naturally-occurring tyrosinase inhibitor which has been clinically used to treat the hyperpigmentation of skin. However, kojic a...
متن کاملSmall Molecule-BIO Accelerates and Enhances Marrow-Derived Mesenchymal Stem Cell in Vitro Chondrogenesis
Background: Hyaline cartilage defects exhibit a major challenge in the field of orthopedic surgery owing to its limited repair capacity. On the other hand, mesenchymal stem cells (MSCs) are regarded as potent cells with a property of cartilage regeneration. We aimed to optimize marrow-derived MSC chondrogenic culture using a small bioactive molecule referred to as BIO. Methods: MSCs from the ma...
متن کاملDevelopment of a Novel Anti-Adhesive Vaccine Against Pseudomonas aeruginosa Targeting the C-terminal Disulfide Loop of the Pilin Protein
The type IV pili (T4P) is a major virulence factor of Pseudomonas aeruginosa (P. aeruginosa) that is associated with primary adhesion, biofilm formation and twitching motility. This study focuses on the introduction of a novel biologically active subunit vaccine derived from the disulfide loop (DSL) of P. aeruginosa pilin. We investigated the expression of the novel PilA in-frame with pET26a ve...
متن کاملDiscovery of Novel Peptidomimetics for Brain-Derived Neurotrophic Factor using Phage Display Technology
Brain-Derived Neurotrophic Factor (BDNF) is a neuroprotectant candidate for neurodegenerative diseases. However, there are several clinical concerns about its therapeutic applications. In the current study, we selected BDNF-mimicking small peptides from phage-displayed peptide library as alternative molecules to the clinical challenges. The peptide library was screened against BDNF receptor (Ne...
متن کاملAnalytical Solution of Stress Field in Adhesively Bonded Composite Single-Lap Joints Under Mechanical Loadings
In this paper, considering an adhesively bonded composite single-lap joint, a novel approach is presented to predict the peel and shear stress distributions of the adhesive layer for an ASTM standard test sample. In current method, the equilibrium equations are derived using the energy method and based on the Timoshenko’s beam theory. Two solution procedures then are discussed, one of which rep...
متن کامل